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Published on: November 23, 2017
[Effects of parthenolide on the activity and expression of urokinase type plasminogen activator in PGCL3 cells]
Dongming Zhong1, Rufang Zhu, Maoyao Zhong
1Zhanjiang Institute of Occupational Disease Prevention, Guangdong Zhanjiang 524018.
Objective:
To study the effect of Parthenolide on the activity and expression of urokinase type plasminogen activator (uPA) on PGCL3 cells and to investigate the antitumor mechanisms of Parthenolide.
Methods:
MTT assay was used to determine the growth inhibition by Parthenolide in PGCL3 cells; Effect of Parthenolide on the activity of uPA secreted by PGCL3 cells was measured by chromogenic substrate assay;The expression level of uPA protein was assessed by Western blot analysis.
Results:
Parthenolide could inhibit proliferation of PGCL3 cells after 24h treatment, the IC50 value was 17.60 micromol/L; Parthenolide reduced significantly the activity of uPA secreted by PGCL3 cells and down-regulated the expression level of uPA protein.
Conclusion:
The antitumor activity of Parthenolide is involved in the activity and expression of urokinase type plasminogen activator.
