Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Phospholipid vesicles promote human hemoglobin oxidation.

C C LaBrake1, L W Fung

  • 1Department of Chemistry, Loyola University, Chicago, Illinois 60626.

The Journal of Biological Chemistry
|August 15, 1992
PubMed
Summary

Lipid vesicles accelerate human hemoglobin oxidation, with smaller vesicles showing a greater effect. This occurs even without lipid peroxidation, suggesting lipid properties influence hemoglobin

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hirayama disease in a 17-year-old Chinese man.

Singapore medical journal·2014
Same author

Studies of the erythrocyte spectrin tetramerization region.

Cellular & molecular biology letters·2001
Same author

alpha beta Spectrin coiled coil association at the tetramerization site.

Biochemistry·2001
Same author

Laboratory method to study mutational effects on human erythrocyte spectrin tetramerization.

American journal of hematology·2001
Same author

NMR analysis of secondary structure and dynamics of a recombinant peptide from the N-terminal region of human erythroid alpha-spectrin.

FEBS letters·2000
Same author

Ascorbate levels in red blood cells and urine in patients with sickle cell anemia.

American journal of hematology·2000

Area of Science:

  • Biochemistry
  • Physical Chemistry

Background:

  • Human hemoglobin (Hb) is prone to autoxidation.
  • Lipid peroxidation can affect Hb stability.
  • The role of lipid vesicles in Hb oxidation is not fully understood.

Purpose of the Study:

  • To investigate the effect of lipid vesicles on human hemoglobin oxidation kinetics.
  • To determine the influence of vesicle size and lipid composition on Hb oxidation.
  • To elucidate the mechanism by which lipids affect Hb oxidation.

Main Methods:

  • Studied heme oxidation kinetics of purified human hemoglobin.
  • Utilized dipalmitoyl phosphatidylcholine and bovine brain phosphatidylserine lipid vesicles.
  • Determined pseudo first-order rate constants for Hb oxidation species formation.

Main Results:

  • Lipid vesicles significantly enhanced Hb oxidation, with small unilamellar vesicles (SUVs) having a larger effect than large unilamellar vesicles (LUVs).
  • Oxidation rate constants increased linearly with the lipid-to-hemoglobin molar ratio.
  • Enhanced oxidation was inhibited by catalase but not by butylated hydroxytoluene, indicating a role for reactive oxygen species.

Conclusions:

  • Lipid vesicles, independent of lipid peroxidation, promote hemoglobin oxidation.
  • Vesicle surface properties such as curvature, surface energy, and hydrophobicity likely contribute to enhanced Hb oxidation.
  • Catalase inhibition suggests a mechanism involving hydrogen peroxide generation.

Related Experiment Videos