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Multiple ligand applications in high-performance immunoaffinity chromatography
1Rainin Instrument Company, Berkeley, CA 94710.
A mixture of antibodies specific for albumin and transferrin, immobilized onto a single support, was used for the simultaneous extraction of albumin and transferrin from immunoglobulin G by high-performance immunoaffinity chromatography. The affinity column was coupled to a strong cation exchanger in order to monitor the success of the extraction and to demonstrate the compatibility of the two chromatographic modes. Coupling of non-affinity chromatography with multiple ligand affinity chromatography is discussed as an alternative to positive affinity chromatography for protein purification.
A mixture of antibodies specific for albumin and transferrin, immobilized onto a single support, was used for the simultaneous extraction of albumin and transferrin from immunoglobulin G by high-performance immunoaffinity chromatography. The affinity column was coupled to a strong cation exchanger in order to monitor the success of the extraction and to demonstrate the compatibility of the two chromatographic modes. Coupling of non-affinity chromatography with multiple ligand affinity chromatography is discussed as an alternative to positive affinity chromatography for protein purification.