Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Eosinophil phenotypes in bullous pemphigoid.

S Tsuda1, M Miyasato, K Iryo

  • 1Department of Dermatology, Kurume University School of Medicine, Japan.

The Journal of Dermatology
|May 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification and isolation of common and tissue-specific geranylgeranylated gamma subunits of guanine-nucleotide-binding regulatory proteins in various tissues.

European journal of biochemistry·1992
Same author

Extra-adrenal pheochromocytoma-ganglioneuroma. A case report.

Pathology, research and practice·1992
Same author

Developmental regulation of cytochrome P-450 of intrasplenically transplanted fetal hepatocytes in spontaneously hypertensive rats.

Transplantation proceedings·1992
Same author

Manganese-containing superoxide dismutase in blood and urine during open-heart surgery.

Japanese circulation journal·1992
Same author

A Japanese pedigree of autosomal dominant congenital stationary night blindness with variable expressivity.

Ophthalmic paediatrics and genetics·1992
Same author

Effects of daily physical activity on insulin action in the elderly.

Journal of applied physiology (Bethesda, Md. : 1985)·1992

Activated, hypodense eosinophils are found in bullous pemphigoid (BP) patients. These eosinophils infiltrate skin lesions and may cause blistering by damaging basal keratinocytes through granule protein release.

Area of Science:

  • Immunology
  • Dermatology
  • Cell Biology

Background:

  • Bullous pemphigoid (BP) is an autoimmune blistering disease.
  • The role of eosinophils in BP pathogenesis is not fully understood.

Purpose of the Study:

  • To investigate eosinophil phenotypes in bullous pemphigoid patients.
  • To determine the activation state and localization of eosinophils in BP lesions.

Main Methods:

  • Density gradient fractionation of peripheral blood eosinophils.
  • Immunocytochemistry using EG2 antibody for eosinophil cationic protein (ECP).
  • Ultrastructural analysis of eosinophil morphology.
  • Analysis of eosinophil infiltration in skin biopsies and bullous fluids.

Main Results:

Related Experiment Videos

  • Two eosinophil phenotypes were identified: normodense and hypodense.
  • BP patients showed increased numbers of hypodense eosinophils in peripheral blood.
  • Hypodense eosinophils expressed high levels of ECP and exhibited features of activation.
  • Activated eosinophils infiltrated the dermo-epidermal junction in BP skin lesions.
  • Higher ECP concentrations were found in bullous fluids compared to sera.

Conclusions:

  • Hypodense eosinophils represent an activated phenotype.
  • Activated eosinophils infiltrate BP skin lesions and degranulate.
  • Eosinophil-derived ECP may contribute to basal keratinocyte damage and dermo-epidermal separation in BP.