Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Versatile expression and secretion vectors for Bacillus subtilis.

Ulf Brockmeier1, Marion Wendorff, Thorsten Eggert

  • 1Institut für Molekulare Enzymtechnologie, Heinrich-Heine Universität Düsseldorf, Forschungszentrum Jülich, D-52426, Jülich, Germany.

Current Microbiology
|February 2, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Deactivation of ceramide de novo synthesis induces cerebral angiogenesis and microvascular remodeling post-ischemia/reperfusion in mice via mechanisms not predominated by extracellular vesicles.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism·2025
Same author

MFN2 and BAG6 Synergistically Protect Against Cerebral Reperfusion Injury by Regulating ROS Levels and Autophagic Flux.

Stroke·2025
Same author

Autophagy hub-protein p62 orchestrates oxidative, endoplasmic reticulum stress, and inflammatory responses post-ischemia, exacerbating stroke outcome.

Redox biology·2025
Same author

Activation of the PERK Branch of the UPR as a Strategy for Improving Outcomes in Acute Ischemic Stroke.

ACS omega·2025
Same author

Acid sphingomyelinase inhibition induces cerebral angiogenesis post-ischemia/reperfusion in an oxidative stress-dependent way and promotes endothelial survival by regulating mitochondrial metabolism.

Cell death & disease·2024
Same author

BAG3 Overexpression Attenuates Ischemic Stroke Injury by Activating Autophagy and Inhibiting Apoptosis.

Stroke·2023
Same journal

Promoter Engineering of the Surfactin Operon Enhances Surfactin Production in the Environmental Strain Bacillus subtilis RI4914.

Current microbiology·2026
Same journal

Staphylococcus aureus CC398 Lineage of the Human Clade Isolated from Bloodstream Infection and Colonization and Spread among Brazilian Patients Hospitalized during the COVID-19 Pandemic.

Current microbiology·2026
Same journal

Evaluation of the Immunogenicity of an Inactivated Vaccine against the Adenovirus Egg Drop Syndrome 1976 in Poultry.

Current microbiology·2026
Same journal

Sphingomonas chlorellae sp. nov., Isolated from Outdoor Photobioreactor Culturing Chlorella sp. HS2 with Soy Sauce Wastewater.

Current microbiology·2026
Same journal

Imported Cutaneous Leishmaniasis in India: Diagnosis and Treatment with Implications for Kala Azar Elimination.

Current microbiology·2026
Same journal

Integrated Screening and Antifungal Potential of Microalgae Extracts Against Botrytis californica.

Current microbiology·2026
See all related articles

Researchers developed new Bacillus subtilis expression vectors, pBSMuL1 and pBSMuL2, to overcome Escherichia coli limitations in heterologous protein production. These vectors facilitate efficient protein overexpression and secretion, simplifying purification.

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Microbial Genetics

Background:

  • Escherichia coli is a common host for heterologous gene expression but presents challenges like inclusion body formation and toxicity.
  • Limitations in E. coli expression systems necessitate alternative hosts for efficient protein production and purification.

Purpose of the Study:

  • To construct and characterize novel expression vectors for Bacillus subtilis.
  • To enable overexpression and secretion of heterologous proteins using Bacillus subtilis as an alternative host.

Main Methods:

  • Construction of two new vectors, pBSMuL1 and pBSMuL2, for Bacillus subtilis.
  • Incorporation of a multiple cloning site, strong constitutive promoters, and a signal sequence for secretion.
  • Demonstration of vector applicability using His-tagged fusion protein expression and purification.

Related Experiment Videos

Main Results:

  • The new vectors offer an enhanced multiple cloning site with 13 unique restriction sites.
  • Vectors feature strong constitutive promoters (one or two) for high-level gene expression.
  • Successful production and purification of heterologous cutinase from Fusarium solani pisi were achieved.

Conclusions:

  • pBSMuL1 and pBSMuL2 are effective tools for heterologous protein overexpression and secretion in Bacillus subtilis.
  • These vectors provide advantages over existing Bacillus expression systems for protein production.
  • The developed system simplifies the detection and purification of recombinant proteins.