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Non-viral heterogeneous sequences at the 5' ends of tomato spotted wilt virus mRNAs
R Kormelink1, F van Poelwijk, D Peters
1Department of Virology, Wageningen, The Netherlands.
The Journal of General Virology
|August 1, 1992
Abstract:
Subgenomic messenger RNAs transcribed from the tomato spotted wilt virus (TSWV) S RNA segment were partially purified from total RNA extracts of TSWV-infected Nicotiana rustica and analysed by primer extension analysis. The data obtained show the presence of non-viral sequences, 12 to 20 nucleotides in length, at the 5' ends of the N and NSs mRNAs, indicating a cap-snatching mechanism for the initiation of transcription. This is the first report of a plant virus using such a mechanism for transcription of the viral genome.