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Related Experiment Videos

Identification of a gene encoding a functional reverse transcriptase within a highly variable locus in the P2-like

Richard Odegrip1, Anders S Nilsson, Elisabeth Haggård-Ljungquist

  • 1Department of Genetics, Microbiology and Toxicology, Stockholm University, S-106 91 Stockholm, Sweden.

Journal of Bacteriology
|February 3, 2006
PubMed
Summary

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P2-like coliphages share highly similar structural genes but differ in horizontally transferred genes. One such region, the TO region, contains diverse sequences, with one product showing reverse transcriptase activity that inhibits T5 phage infection.

Area of Science:

  • Microbiology
  • Virology
  • Genetics

Background:

  • P2-like coliphages are a group of bacteriophages that infect Escherichia coli.
  • These phages exhibit high similarity in their structural genes, suggesting a common ancestry.
  • Evidence suggests horizontal gene transfer events have occurred in certain genomic regions of these phages.

Purpose of the Study:

  • To investigate the genetic diversity within the horizontally transferred regions of P2-like coliphages.
  • To characterize the genetic content and functional properties of the TO region.
  • To determine the impact of TO region genetic variation on phage infectivity.

Main Methods:

  • Comparative genomic analysis of P2-like coliphage sequences.
  • Bioinformatic analysis to identify open reading frames and sequence diversity.

Related Experiment Videos

  • Functional assays to assess reverse transcriptase activity.
  • Infection inhibition assays using phage T5.

    • Main Results:

    • The TO region, a site of presumed horizontal gene transfer, displays significant sequence divergence among P2-like coliphages.
    • Six distinct sequences with high AT content and varying open reading frames were identified within the TO region.
    • The product of one identified gene demonstrated reverse transcriptase activity.
    • This reverse transcriptase activity was found to inhibit the infection of phage T5.

    Conclusions:

    • Horizontal gene transfer has introduced substantial genetic novelty into the TO region of P2-like coliphages.
    • The identified reverse transcriptase in the TO region represents a novel mechanism for phage-host interaction or phage defense.
    • This finding contributes to understanding phage evolution and the dynamics of gene exchange in microbial communities.