Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantification of mtDNA mixtures in forensic evidence material using pyrosequencing.

H Andréasson1, M Nilsson, B Budowle

  • 1Rudbeck Laboratory, Department of Genetics and Pathology, Uppsala University, 75185 Uppsala, Sweden.

International Journal of Legal Medicine
|February 3, 2006
PubMed
Summary

This study introduces a novel pyrosequencing method for accurate mitochondrial DNA (mtDNA) mixture quantification. The assay precisely determines mixture ratios in forensic samples, resolving major and minor DNA components.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

4th DNA Forensic Symposium: Challenges and future directions in Africa.

Forensic science international. Synergy·2024
Same author

Controlled mechanical stimuli reveal novel associations between basil metabolism and sensory quality.

Food chemistry·2022
Same author

Association of whole mtDNA, an NADPH G11914A variant, and haplogroups with high physical performance in an elite military troop.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2021
Same author

Evaluation of InnoTyper® 21 in a sample of Rio de Janeiro population as an alternative forensic panel.

International journal of legal medicine·2017
Same author

Letter to the editor: Are the doors opened to a genetic-based algorithm for personalized resistance training?

Biology of sport·2017
Same author

Global genetic variation of select opiate metabolism genes in self-reported healthy individuals.

The pharmacogenomics journal·2017

Area of Science:

  • Forensic Science
  • Molecular Biology
  • Genetics

Background:

  • Sanger sequencing lacks accuracy for quantifying mitochondrial DNA (mtDNA) mixtures.
  • Accurate quantification is crucial for forensic samples with heteroplasmy or contamination.
  • A need exists for a reliable method to determine mtDNA mixture ratios.

Purpose of the Study:

  • To develop and validate a novel quantification system for mtDNA mixture analysis.
  • To utilize pyrosequencing technology for precise measurement of mtDNA components.
  • To assess the linear relationship between incorporated nucleotides and released light for quantification.

Main Methods:

  • Developed a quantification system for mtDNA mixture analysis using pyrosequencing technology.
  • Evaluated seven variable positions across five PCR fragments in mtDNA control and coding regions.

Related Experiment Videos

  • Quantified single nucleotide polymorphisms (SNPs) to determine mixture ratios.

    • Main Results:

    • Observed a linear relationship between measured and expected mixture ratios for all quantified SNPs.
    • Demonstrated the system's ability to accurately quantify mtDNA components in mixtures.
    • Validated the pyrosequencing approach for mtDNA mixture analysis.

    Conclusions:

    • The developed mtDNA quantification assay is easy to use, fast, and accurate.
    • This method effectively resolves and interprets major and minor mtDNA components in forensic mixtures.
    • Pyrosequencing offers a valuable alternative for accurate mtDNA mixture ratio determination.