Related Experiment Video
Updated: Aug 13, 2026

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean
Published on: December 23, 2017
Nodule-specific host proteins in effective and ineffective root nodules of Pisum sativum
T Bisseling1, C Been, J Klugkist
1Laboratory of Molecular Biology, Agricultural University, De Dreijen 11, 6703 BC Wageningen, The Netherlands.
Abstract:
Nodule-specific root proteins - so called nodulins - were identified in root nodules of pea plants by an immunological assay. Nodulin patterns were examined at different stages of nodule development. About 30 nodulins were detectable during development. Some were preferentially synthesized before nitrogen fixation started, whereas the majority were synthesized concomitantly with leghaemoglobin. Some of the nodulins were located within the peribacteroid membrane. Ineffective Rhizobium strains (a natural nodfix and a pop fix) appeared to be useful in studying the expression of nodulin genes. Synthesis of some nodulins was repressed in ineffective root nodules, indicating that nodulins are essential for the establishment of nitrogen fixation. In both types of ineffective root nodules, leghaemoglobin synthesis was not completely repressed. Low amounts of leghaemoglobin were always detected in young ineffective root nodules whereas in old nodules no leghaemoglobin was present.
More Related Videos
20:01Single-plant, Sterile Microcosms for Nodulation and Growth of the Legume Plant Medicago truncatula with the Rhizobial Symbiont Sinorhizobium meliloti
Published on: October 1, 2013
12:22Transforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii
Published on: August 18, 2019
Related Concept Videos
The Roles of Bacteria and Fungi in Plant Nutrition
Microbe-Plant Interactions
Epiphytes, Parasites, and Carnivores
Inorganic Nitrogen Assimilation
Cell Signaling in Plants