An Agrobacterium-transformed cell culture from the monocot Asparagus officinalis
J P Hernalsteens1, L Thia-Toong, J Schell
1Laboratorium voor Genetische Virologie, Vrije Universiteit Brussel, B-1640 St.-Genesius-Rode,FRG.
Abstract:
Cultured stem fragments from the monocotyledonous plant Asparagus officinalis infected by the oncogenic bacterium Agrobacterium tumefaciens developed tumorous proliferations. This tissue was propagated in vitro on hormone-free culture medium. The T-DNA-encoded markers nopaline and agrocinopine were unambiguously detected in these tissues. The data demonstrate that stable T-DNA transfer as well as expression of T-DNA genes is possible in at least some monocotyledonous plants. This opens new possibilities for plant genetic engineering using the Ti plasmid as a gene vector.
Related Concept Videos
Transgenic Plants
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Plant Tissue Culture
Cell Culture
Bacterial Transformation
Transgenic Organisms


