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Related Experiment Videos

Mating pheromone-like diffusible factor released by Schizosaccharomyces pombe.

Y Fukui1, Y Kaziro, M Yamamoto

  • 1Institute of Medical Science, University of Tokyo, P.O. Takanawa, Tokyo 108, Japan.

The EMBO Journal
|August 1, 1986
PubMed
Summary
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Genes & development·1992

Fission yeast h cells secrete an h-factor that elongates cells under nitrogen-depleted conditions. The S. pombe ras1 gene product may be involved in responding to this mating pheromone.

Area of Science:

  • Cell biology
  • Microbiology
  • Molecular biology

Background:

  • Mating pheromones in the fission yeast Schizosaccharomyces pombe are not fully described.
  • The role of the S. pombe ras1 gene, a homolog of mammalian ras oncogenes, in mating is unclear.

Purpose of the Study:

  • To investigate the existence and function of diffusible factors secreted by h cells in Schizosaccharomyces pombe.
  • To explore the potential role of the S. pombe ras1 gene product in pheromone response.

Main Methods:

  • Observation of cell elongation in response to secreted factors under nitrogen-depleted conditions.
  • Analysis of factor secretion and response in wild-type and hras1 mutant cells.

Main Results:

  • A diffusible factor, termed h-factor, is secreted by h cells and induces cell elongation in S. pombe under nitrogen-depleted conditions.

Related Experiment Videos

  • While hras1 cells secrete h-factor, they have lost the ability to respond to it.

    • Conclusions:

    • The secreted h-factor plays a role in the mating process of S. pombe.
    • The S. pombe ras1 gene product is likely involved in the mechanism of responding to mating pheromones.