Related Experiment Video
Updated: Jul 24, 2026

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
Published on: September 11, 2017
Functional analysis of the transcriptional control regions of the copia transposable element
J H Sinclair1, J F Burke, D Ish-Horowicz
1School of Biological Sciences, University of Sussex, Falmer, Sussex, UK.
Abstract:
The introduction of copia-based vectors in Drosophila hydei cells results in their high-level transient expression and the subsequent establishment of stably transformed cell lines containing multiple copies of vector integrated into host genomic DNA. Using transformation frequency and transient expression analysis as assays of promoter strength, we have defined the regions of copia essential for expression. We find that the essential sequences reside within the long terminal repeat, but 3' to the site of initiation of copia RNA. Deletion of the consensus enhancer-like sequences from copia appears to have no effect on vector expression.
Related Concept Videos
Overview of Transposition and Recombination
DNA-only Transposons
The donor site from where the transposon is excised is either degraded or...
LTR Retrotransposons
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
RNA Polymerase II Accessory Proteins
Cis-regulatory Sequences
Cis-regulatory Sequences

