Related Experiment Video
Updated: Aug 13, 2026

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction
Published on: August 9, 2011
Three-dimensional structure of the regular surface glycoprotein layer of Halobacterium volcanii from the Dead Sea
M Kessel1, I Wildhaber, S Cohen
1Department of Membrane and Ultrastructure Research, Hadassah Medical School, The Hebrew University, PO Box 1172, Jerusalem 91-010, Israel.
Abstract:
A three-dimensional reconstruction from electron micrographs of negatively stained cell envelopes of Halobacterium volcanii has revealed the structure of the surface glycoprotein to a resolution of 2 nm. The glycoprotein is arranged on a p6 lattice with a lattice constant of 16.8 nm. It forms 4.5 nm high, dome-shaped, morphological complexes with a narrow pore at the apex opening into a ;funnel' towards the cell membrane. The polarity of the structure was derived from freeze-etching experiments and ;edge' views. Six radial protrusions emanate from each morphological complex and join around the 3-fold axis to provide lateral connectivity. Using the primary structure of the surface glycoprotein of the closely related species Halobacterium halobium (Lechner and Sumper, 1987) and the cell envelope profile from a previous X-ray analysis of the same species (Blaurock et al., 1976) we have integrated our reconstruction into a model of halobacterial cell envelope.
More Related Videos
09:12Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System
Published on: March 10, 2020
06:33Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
Published on: October 29, 2019
Related Concept Videos
Surface Appendages of Archaea
Archaeal Cell Wall
Outer Layers of the Cell Envelope
Plasma Membrane in Bacteria and Archaea
Newman Projections
The organic molecules rotate across the single bonds leading to numerous temporary three-dimensional structures of varying energy known as conformers.
Viral Structure