Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Maize streak virus genes essential for systemic spread and symptom development.

S G Lazarowitz1, A J Pinder, V D Damsteegt

  • 1Department of Embryology, The Carnegie Institution of Washington, 115 W, University Pkwy, Baltimore, MD 21210, USA.

The EMBO Journal
|April 1, 1989
PubMed
Summary

Maize streak virus (MSV) capsid and a specific ORF are crucial for spreading disease in plants, though not for viral replication. This research identifies key MSV genes for systemic infection and symptom development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prunus tomentosa as a Diagnostic Host for Detection of Plum Pox Virus and Other Prunus Viruses.

Plant disease·2019
Same author

Occurrence and Partial Characterization of a New Mechanically Transmissible Virus in Mashua from the Ecuadorian Highlands.

Plant disease·2019
Same author

Molecular Analyses of Citrus tristeza virus Subisolates Separated by Aphid Transmission.

Plant disease·2019
Same author

Transmission of the Citrus Variegated Chlorosis Bacterium Xylella fastidiosa with the Sharpshooter Oncometopia nigricans.

Plant disease·2019
Same author

Histology of Sweet Orange Stem Pitting Caused by an Australian Isolate of Citrus tristeza virus.

Plant disease·2019
Same author

Prunus Host Range of Plum pox virus (PPV) in the United States by Aphid and Graft Inoculation.

Plant disease·2019

Area of Science:

  • Plant virology
  • Molecular biology
  • Genetics

Background:

  • Single-component geminiviruses, like maize streak virus (MSV), possess a small (~2.7 kb) single-stranded circular DNA genome.
  • Despite limited coding capacity, MSV causes significant monocot diseases, including chlorosis and stunting.

Purpose of the Study:

  • To identify viral gene functions essential for systemic spread and symptom development in MSV-infected maize.
  • To delineate the genomic regions of MSV required for autonomous replication.

Main Methods:

  • Site-directed mutagenesis and gene replacement were used to create MSV mutants.
  • Agrobacterium-mediated inoculation of maize seedlings followed by analysis of viral DNA excision via homologous recombination.
  • Analysis of viral gene functions, including the capsid gene and a specific ORF, for replication and systemic spread.

Related Experiment Videos

Main Results:

  • The MSV capsid gene is dispensable for replication but essential for systemic spread and disease development.
  • A specific ORF upstream of the capsid gene is also essential for systemic spread, but not replication.
  • MSV sequences supporting autonomous replication were localized to a 1.7 kb segment containing intergenic regions and complementary strand ORFs.
  • Mutant viruses unable to spread systemically induced chlorotic patterns on leaves, resembling maize developmental mutants.

Conclusions:

  • The capsid gene and a specific upstream ORF are critical for MSV systemic infection and disease progression in maize.
  • The identified 1.7 kb genomic segment is sufficient for MSV autonomous replication.
  • MSV infection can phenocopy maize developmental mutations, suggesting shared molecular pathways.