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Updated: Aug 13, 2026

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
Published on: February 14, 2020
Transpositional behavior of the maize En/Spm element in transgenic tobacco
Abstract:
The transposable element Enhancer (En/Spm) of Zea mays encodes the functions necessary for transposition of members of the En-I family of elements. En constructs were introduced into tobacco and transposition was demonstrated by cloning and DNA sequence analysis. Transcriptional analysis of En transgenic plants revealed 2.5 and 6 kb En homologous transcripts similar in size to those found in maize, in addition to aberrant splice products. Progeny analysis of a primary transformant indicates En transpositional activity after meiosis. In these progeny somatic variegation, at the NPT II gene, was demonstrated to be caused by En excision. Two re-insertions of En were analyzed and found to reside in unique copy DNA while another was found in middle repetitive DNA.
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