Beta-galactosidase catalyzed selective galactosylation of aromatic compounds
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Updated: Aug 11, 2026

Synthesis of Indoxyl-glycosides for Detection of Glycosidase Activities
Published on: May 27, 2015
Nicolas Bridiau1, Selma Taboubi, Nejib Marzouki
1Laboratoire de Biotechnologie et de Chimie Bio-organique, CNRS FRE 2766, Université de La Rochelle, Avenue Michel Crépeau, 17042 La Rochelle, France.
Abstract:
A new approach to galacto-oligosaccharides and galacto-conjugates synthesis performed by the beta-galactosidase from Kluyveromyces lactis is reported. The enzymatic galactosylation of eight kinds of adsorbed aromatic primary alcohols, in particular the two drugs guaifenesin and chlorphenesin, gave the corresponding beta-D-galacto-pyranosides in yields ranging between approximately 10% and 96%. For the first time, we have showed that the adsorption of acceptor substrates onto solid supports such as silica gel influences the yield and the selectivity of galacto-conjugates synthesis. In particular, we observed that adsorption of acceptor favored the synthesis of digalactosylated compounds.
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