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Related Experiment Videos

Cytometry on a chip: cellular phenotypic and functional analysis using grating-coupled surface plasmon resonance.

Guang-bi Jin1, Darryn W Unfricht, Salvador M Fernandez

  • 1Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269-3125, United States.

Biosensors & Bioelectronics
|February 4, 2006
PubMed
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Grating-coupled surface plasmon resonance imaging (GCSPRI) accurately assesses cell phenotype and function. This method offers a fast, powerful way to simultaneously analyze up to 400 targets, outperforming traditional flow cytometry.

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Cell Biology

Background:

  • Surface plasmon resonance (SPR) is a label-free optical technique.
  • Assessing cell phenotype and function requires sensitive and high-throughput methods.
  • Existing methods like flow cytometry have limitations in simultaneous phenotype and function analysis.

Purpose of the Study:

  • To demonstrate the capability of Grating-Coupled Surface Plasmon Resonance Imaging (GCSPRI) for cell and protein analysis.
  • To compare GCSPRI performance against traditional flow cytometry.
  • To highlight GCSPRI as a tool for simultaneous assessment of cell phenotype and function.

Main Methods:

  • Utilized GCSPRI with antibodies immobilized on a gold-coated sensor chip.
  • Analyzed refractive index changes by monitoring shifts in the Grating-Coupled Surface Plasmon Resonance (GCSPR) angle.

Related Experiment Videos

  • Performed parallel assessment of up to 400 antibody regions.
  • Compared GCSPRI results with data from a flow cytometry system.

    • Main Results:

    • GCSPRI successfully identified cells and proteins of interest.
    • Demonstrated the ability to image cells during capture.
    • Showcased the multiplexed format for parallel analysis.
    • GCSPRI results were comparable to flow cytometry data.

    Conclusions:

    • GCSPRI is a highly accurate method for assessing cell phenotype and function.
    • The technology enables fast, simultaneous analysis of multiple targets.
    • GCSPRI offers a powerful alternative to traditional methods for cell and protein analysis.