Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bilayer structure in phospholipid-cytochrome c model membranes.

Shui-Pong-Van, O H Griffith

    The Journal of Membrane Biology
    |January 1, 1975
    PubMed
    Summary

    Extrinsic proteins like cytochrome c show minimal impact on lipid bilayers, unlike intrinsic proteins. This suggests extrinsic proteins do not interfere with boundary lipid measurements in biological membranes.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Advantages and limitations of spin labeling in quantitating protein-lipid associations.

    Biophysical journal·2009
    Same author

    A catalytic diad involved in substrate-assisted catalysis: NMR study of hydrogen bonding and dynamics at the active site of phosphatidylinositol-specific phospholipase C.

    Biochemistry·2001
    Same author

    Sensitive fluorescent quantitation of myo-inositol 1,2-cyclic phosphate and myo-inositol 1-phosphate by high-performance thin-layer chromatography.

    Journal of chromatography. B, Biomedical sciences and applications·2001
    Same author

    Synthesis of fluorogenic substrates for continuous assay of phosphatidylinositol-specific phospholipase C.

    Bioconjugate chemistry·2001
    Same author

    High-molecular-weight serum protein complexes differentially promote cell migration and the focal adhesion localization of the urokinase receptor in human glioma cells.

    Experimental cell research·2000
    Same author

    Bacterial phosphatidylinositol-specific phospholipase C: structure, function, and interaction with lipids.

    Biochimica et biophysica acta·1999

    Area of Science:

    • Membrane biophysics
    • Lipid-protein interactions
    • Spectroscopic analysis of biological systems

    Background:

    • Lipid-protein interactions are crucial for biological membrane function.
    • Intrinsic and extrinsic proteins interact differently with lipid bilayers.
    • Previous studies on intrinsic proteins (cytochrome oxidase) revealed boundary lipid layers.

    Purpose of the Study:

    • To investigate the interaction of an extrinsic protein (cytochrome c) with a phospholipid bilayer.
    • To compare the effects of extrinsic versus intrinsic proteins on lipid bilayer behavior.
    • To determine if extrinsic proteins interfere with boundary lipid measurements.

    Main Methods:

    • Utilizing lipid spin labels to study phospholipid bilayers.
    • Complexing cytochrome c with a cardiolipin/lecithin mixture.
    • Analyzing Electron Spin Resonance (ESR) spectra based on four criteria: fluidity gradient, spectral anisotropy, hydration response, and polarity profile.

    Main Results:

    • Phospholipids in the presence and absence of cytochrome c exhibited typical bilayer behavior.
    • Electron Spin Resonance (ESR) spectral changes induced by cytochrome c were minimal.
    • Observed effects were in stark contrast to those seen with intrinsic proteins.

    Conclusions:

    • Cytochrome c likely binds electrostatically to charged phospholipid groups.
    • Extrinsic proteins do not significantly alter lipid bilayer structure or dynamics.
    • Extrinsic proteins are unlikely to interfere with boundary lipid measurements in biological membranes.

    Related Experiment Videos