Search research articles

Search research articles

Home
Immunochemical Testing Of Individuals Positive For Guaiac Faecal Occult Blood Test In A Screening Programme For Colorectal Cancer: An Observational Study.

Home

Related Concept Videos

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Immunochemical Testing Of Individuals Positive For Guaiac Faecal Occult Blood Test In A Screening Programme For Colorectal Cancer: An Observational Study.

Related Experiment Videos

Immunochemical testing of individuals positive for guaiac faecal occult blood test in a screening programme for

Callum G Fraser¹, Catriona M Matthew, N Ashley G Mowat

¹Department of Biochemical Medicine, Ninewells Hospital and Medical School, Dundee, Scotland, UK. callum.fraser@tuht.scot.nhs.uk

The Lancet. Oncology

|February 4, 2006

View abstract on PubMed

Summary

This summary is machine-generated.

Using immunochemical FOBT after a positive guaiac FOBT test can significantly reduce false positives in colorectal cancer screening. This improves the selection of individuals who truly need colonoscopy for accurate diagnosis.

Related Experiment Videos

Area of Science:

Gastroenterology
Oncology
Medical Diagnostics

Background:

Guaiac-based faecal occult blood tests (FOBT) are used for colorectal cancer screening, reducing mortality.
However, guaiac FOBT has imperfect specificity, leading to many false positives requiring colonoscopy.
A sensitive immunochemical FOBT may better select individuals positive for guaiac FOBT who require colonoscopy.

Purpose of the Study:

To evaluate if immunochemical FOBT can improve the selection of individuals for colonoscopy after a positive guaiac FOBT result in colorectal cancer screening.
To assess the diagnostic accuracy of immunochemical FOBT in identifying colorectal neoplasia in a guaiac FOBT-positive population.

Main Methods:

Individuals with a positive guaiac FOBT in a pilot screening study provided duplicate stool samples for immunochemical FOBT.
Results were categorized as negative/negative (N/N), negative/positive (N/P), or positive/positive (P/P).
Immunochemical FOBT results were compared with colonoscopy findings to calculate sensitivity, specificity, and likelihood ratios for cancer and high-risk adenomatous polyps.

Main Results:

Of 801 participants returning samples, 62% had both samples positive (P/P) on immunochemical FOBT.
The P/P group showed a significantly higher detection rate for colorectal cancer (8% vs. <1%) and high-risk adenomatous polyps (39% vs. 16-19%) compared to N/N and N/P groups.
The odds ratio for P/P being associated with cancer was 7.57, and with high-risk adenomatous polyps was 3.11.

Conclusions:

Immunochemical FOBT is effective in identifying individuals with a higher likelihood of colorectal cancer or advanced polyps after a positive guaiac FOBT.
This approach can substantially decrease the number of false positives in colorectal cancer screening programs.
Utilizing immunochemical FOBT can optimize colonoscopy referrals, improving screening efficiency.