Related Experiment Video
Updated: Jul 19, 2026

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
Published on: September 5, 2013
High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study
M Aires-de-Sousa1, K Boye, H de Lencastre
1Department of Periodontology, University Hospital Münster, Waldeyerstrasse 30, D-48149 Münster, Germany.
Abstract:
Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.
Related Concept Videos
Modern Molecular Taxonomy
Applications of Molecular Taxonomy
Automated Microbial Diagnostics

