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Human mast cells undergo TRAIL-induced apoptosis
Beata Berent-Maoz1, Adrian M Piliponsky, Isabelle Daigle
1Department of Pharmacology, School of Pharmacy, The Hebrew University of Jerusalem, Israel.
Journal of Immunology (Baltimore, Md. : 1950)
|February 4, 2006
Summary
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) can induce apoptosis in mast cells (MCs). This pathway may regulate MC survival and reduce hyperplasia in inflammatory conditions.
Area of Science:
- Immunology
- Cell Biology
Background:
- Mast cells (MCs) are crucial in allergic and inflammatory diseases, often exhibiting hyperplasia.
- Stem cell factor (SCF) is the primary regulator of human MC growth and survival.
- The role of extrinsic apoptosis pathways in regulating MC numbers is largely unexplored.
Purpose of the Study:
- To investigate the expression and function of death receptors, specifically Fas ligand and TRAIL receptors, in human mast cells.
- To determine if these receptors mediate apoptosis in mast cells.
Main Methods:
- Examined the expression of Fas and TRAIL receptors in human MC lines (HMC-1, CBMC) and human lung-derived MCs.
- Activated TRAIL receptors to assess their functional role in apoptosis.
- Investigated the effect of IgE-dependent activation on TRAIL-mediated apoptosis susceptibility in CBMC.
Main Results:
- Human MC lines and lung-derived MCs expressed Fas and TRAIL receptors, with CBMC expressing only TRAIL-R.
- Activation of TRAIL-R triggered caspase 3-dependent apoptosis in CBMC and HMC-1.
- IgE-dependent activation enhanced the susceptibility of CBMC to TRAIL-induced apoptosis.
Conclusions:
- TRAIL-mediated apoptosis represents a potential mechanism for regulating mast cell survival in vivo.
- This pathway may play a role in down-regulating mast cell hyperplasia in pathological conditions.