Related Experiment Video
Updated: Aug 11, 2026

08:05
Assaying Protein Kinase Activity with Radiolabeled ATP
Published on: May 26, 2017
NMR assignment of rat Raf kinase inhibitor protein
Matthew C Clark1, Dan McElheny, John Wojcik
1Department of Neurobiology, Pharmacology and Physiology, Ben May Institute for Cancer Research, Chicago, Illinois 60637, USA.
Journal of Biomolecular NMR
|February 4, 2006
Abstract
No abstract available in PubMed .
More Related Videos
Related Concept Videos
MAPK Signaling Cascades
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
PI3K/mTOR/AKT Signaling Pathway
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a rapamycin-insensitive companion...

