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Updated: Aug 11, 2026
Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation
Published on: September 6, 2017
[Sequence analysis of a novel HLA-DRB1 allele, DRB1 * 1212]
Fa-ming Zhu1, Wei Zhang, Qin-feng Lu
1Blood Center of Zhejiang Province, Hangzhou, Zhejiang 310006, P.R. China.
Objective:
To investigate the molecular genetics basis for HLA novel allele HLA-DRB1*1212 in Chinese population.
Methods:
Genomic DNA was extracted from whole blood by salting-out method. HLA-DRB1 gene exon 2 was amplified by PCR with group-specific primers from genomic DNA. PCR products were cut back from agarose gels and purified to sequence directly. The polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSO) was performed to confirm the mutations which were detected by sequencing in this study.
Results:
The sequencing results showed HLA-DRB1 alleles of the proband as DRB1*090102 and the novel allele. The sequences of the novel allele have been submitted to GenBank (AY899825). Through BLAST analysis, the novel allele was found to be different from DRB1*120101 at position 199A-->C in exon 2, that results in an amino acid change from Ile to Leu at codon 67.
Conclusion:
This allele is a novel and has been officially named as DRB1*1212 by the WHO Nomenclature Committee.
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