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Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE)
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Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE)

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Detection of low-molecular weight allergens resolved on two-dimensional electrophoresis with acid-urea polyacrylamide

Kazumi Kitta1, Mayumi Ohnishi-Kameyama, Tatsuya Moriyama

  • 1National Food Research Institute, Research Planning and Coordination Division, Tsukuba, Ibaraki 305-8642, Japan. kaz@nfri.affrc.go.jp

Analytical Biochemistry
|February 7, 2006
PubMed

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DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
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SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
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Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
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Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
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