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Related Experiment Videos

Mapping nucleolar localization sequences of 1A6/DRIM.

Jiangying Liu1, Xiaojuan Du, Yang Ke

  • 1Beijing Institute for Cancer Research, School of Oncology, Peking University, No. 52 Fu-Cheng Rd, Hai-Dian District, Beijing 100036, PR China.

FEBS Letters
|February 7, 2006
PubMed
Summary

Human 1A6/downregulated in metastasis (DRIM) protein accumulates in the nucleolus via a C-terminal region. This region contains essential basic amino acid clusters, overlapping with its nuclear localization signal.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Protein Localization

Background:

  • Human 1A6/downregulated in metastasis (DRIM) is a nucleolar protein featuring HEAT-repeat motifs.
  • Its yeast homolog, Utp20, is involved in 18S ribosomal RNA processing within the small subunit processome.

Purpose of the Study:

  • To identify the specific sequence responsible for the nucleolar accumulation of human 1A6/DRIM.
  • To elucidate the functional elements within this region critical for localization.

Main Methods:

  • Utilized green fluorescent protein (GFP) as a fusion protein marker.
  • Performed deletion sequence analysis to map the nucleolar localization region.
  • Identified essential amino acid clusters within the identified region.

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Main Results:

  • A single region between amino acids 2744 and 2761 at the C-terminus of 1A6/DRIM mediates nucleolar accumulation.
  • Two basic amino acid clusters within this C-terminal region are crucial for this localization.
  • The identified nucleolar accumulation sequence overlaps with the predicted nuclear localization signal of 1A6/DRIM.

Conclusions:

  • The C-terminal region of 1A6/DRIM, containing specific basic amino acid clusters, is essential for its localization to the nucleolus.
  • These findings provide insight into the molecular mechanisms governing nucleolar protein targeting.
  • The overlap with the nuclear localization signal suggests a potential dual role or sequential targeting mechanism.