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Equine herpesvirus 1 sequence near the left terminus codes for two open reading frames.
R R Yalamanchili1, B Raengsakulrach, D J O'Callaghan
1Department of Microbiology and Immunology, Louisiana State University Medical Center, Shreveport 71130-3932.
Virus Research
|March 1, 1991
Summary
Researchers sequenced equine herpesvirus 1 (EHV-1) DNA, identifying two novel open reading frames (ORFs) homologous to varicella-zoster virus genes. This EHV-1 sequence is crucial for generating defective-interfering (DI) particles.
Area of Science:
- Virology
- Molecular Biology
- Genomics
Background:
- Equine herpesvirus 1 (EHV-1) shares genomic terminal homology with other herpesviruses.
- Previous studies established the sequence of EHV-1 genomic termini.
Purpose of the Study:
- To determine the nucleotide sequence adjacent to the left terminus of the EHV-1 genome.
- To identify potential coding regions and their functional significance within this newly sequenced segment.
Main Methods:
- DNA sequencing of the EHV-1 genome.
- Bioinformatic analysis to identify open reading frames (ORFs).
- Comparative genomics to identify homologous sequences in other herpesviruses.
Main Results:
- The nucleotide sequence from map units 0.0087 to 0.0237 of the EHV-1 genome was determined.
- Two ORFs were identified within this region, showing homology to ORF2 and ORF3 of the varicella-zoster virus genome.
- A segment involved in the generation of EHV-1 defective-interfering (DI) particles was identified.
Conclusions:
- The newly sequenced region of the EHV-1 genome contains functionally significant ORFs homologous to those in other herpesviruses.
- This sequence plays a role in the generation of EHV-1 DI particles through intragenomic recombination.