Related Experiment Video
Updated: Aug 11, 2026

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants
Published on: May 4, 2018
[Reservoirs and transmission of Pseudomonas aeruginosa in intensive care unit]
A Lashéras1, O Guisset, H Boulestreau
1Service d'hygiène hospitalière, hôpital Pellegrin, place Amélie-Raba-Léon, 33076 Bordeaux cedex, France. agnes.lasheras@chu-bordeaux.fr
Objective:
The authors had for aim to study reservoirs and transmission of Pseudomonas aeruginosa in an intensive care unit.
Design:
A 6-month prospective descriptive study was made on water samples, samples from hands of health care workers, and clinical samples. P. aeruginosa strains were compared by pulsed-field gel electrophoresis.
Results:
Among the 211 patients hospitalized during the study, 14 (6.6%) were infected by P. aeruginosa. Out of 494 water samples, 80 were contaminated by P. aeruginosa. The regularly disinfected water taps were more rarely contaminated than the others (P<10(-5)). Out of 140 hand samples, one showed contamination from an infected patient.
Conclusions:
aeruginosa cross transmission was observed during this study. We should follow strict hygienic precautions such as wearing gloves and performing thorough alcoholic rub disinfection. Water taps are often contaminated and require regular disinfection.
Insights
Pseudomonas aeruginosa transmission was observed in an intensive care unit. Strict hand hygiene and regular disinfection of water taps are crucial to prevent the spread of this opportunistic pathogen.
Area of Science:
- Infectious Diseases
- Microbiology
- Hospital Epidemiology
Background:
- Pseudomonas aeruginosa is a significant nosocomial pathogen.
- Intensive care units (ICUs) are high-risk environments for healthcare-associated infections.
- Understanding reservoirs and transmission routes is vital for infection control.
Purpose of the Study:
- To investigate the reservoirs and transmission of Pseudomonas aeruginosa in an ICU setting.
- To identify potential sources of P. aeruginosa contamination within the hospital environment.
Main Methods:
- A 6-month prospective descriptive study was conducted.
- Samples were collected from water sources, healthcare worker hands, and patients.
- P. aeruginosa strains were analyzed using pulsed-field gel electrophoresis (PFGE).
Main Results:
- 14 out of 211 patients (6.6%) acquired P. aeruginosa infections.
- 80 out of 494 water samples (16.2%) were contaminated with P. aeruginosa.
- Regularly disinfected water taps showed significantly lower contamination rates compared to others (P<10(-5)).
- One healthcare worker hand sample was contaminated, linked to an infected patient.
Conclusions:
- Pseudomonas aeruginosa cross-transmission occurred within the ICU.
- Strict adherence to hand hygiene, including glove use and alcohol-based hand rubs, is essential.
- Water taps represent a potential reservoir and require consistent disinfection protocols.
Related Concept Videos
Pneumonia I: Introduction
Pneumonia I: Introduction
Risk Factors
Various factors influence the likelihood of developing pneumonia. Age plays a crucial role, with infants, children under two, and individuals over 65 at increased risk due to their...
Reservoir of Infection
Transmission-based Precautions II: Airborne and Protective Environment
Airborne precautions:
Use airborne precautions when treating patients known or suspected to have diseases that spread through the air—for example, tuberculosis or measles. These organisms are present in smaller droplets expelled by an infected person and...
Healthcare Associated Infections II: Preventive Measures
The best practices for preventing healthcare-associated infections include hand hygiene, patient risk...
Pneumonia V: Nursing management and Prevention
The nurse must practice strict medical asepsis and adhere to infection control guidelines to minimize healthcare-associated infections.
Enhance airway patency
Position the patient correctly to facilitate drainage of the affected lung segments. Manual or mechanical percussion and vibration can also be employed.

