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Modulatory role of grape seed extract on age-related oxidative DNA damage in central nervous system of rats
Muthaiya Balu1, Purushotham Sangeetha, Ganesan Murali
1Department of Medical Biochemistry, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600 113, India.
Abstract:
Aging is the accumulation of diverse deleterious changes in the cells and tissues leading to increased risk of diseases. Oxidative stress is considered as a major risk factor and contributes to age related increase in DNA oxidation and DNA protein cross-links in central nervous system during aging. In the present study, we have evaluated the salubrious role of grape seed extract on accumulation of oxidative DNA damage products such as 8-OHdG and DNA protein cross-links in aged rats. Male albino rats of Wistar strain were divided into four groups: Group I, young control rats; Group II, young rats treated with grape seed extract (100 mg/kg b.wt.) for 30 days; Group III, aged control rats; Group IV, aged rats supplemented with grape seed extract (100 mg/kg b.wt.) for 30 days. Our results, thus, revealed that grape seed extract has inhibiting effect on the accumulation of age-related oxidative DNA damages in spinal cord and in various brain regions such as cerebral cortex, striatum and hippocampus.
Insights
Grape seed extract can inhibit age-related oxidative DNA damage in the brain and spinal cord of rats. This finding suggests a potential therapeutic role for grape seed extract in mitigating aging-associated neurodegeneration.
Area of Science:
- Gerontology
- Neuroscience
- Molecular Biology
Background:
- Aging involves cellular and tissue damage, increasing disease risk.
- Oxidative stress is a key factor in age-related DNA damage, particularly in the central nervous system.
- Specific markers of oxidative DNA damage include 8-hydroxy-2'-deoxyguanosine (8-OHdG) and DNA-protein cross-links.
Purpose of the Study:
- To investigate the protective effects of grape seed extract (GSE) against oxidative DNA damage in aged rats.
- To evaluate GSE's impact on the accumulation of 8-OHdG and DNA-protein cross-links in the aging central nervous system.
Main Methods:
- Wistar rats were divided into four groups: young control, young GSE-treated, aged control, and aged GSE-treated.
- Aged rats received GSE (100 mg/kg body weight) daily for 30 days.
- Oxidative DNA damage markers (8-OHdG, DNA-protein cross-links) were assessed in spinal cord and brain regions (cerebral cortex, striatum, hippocampus).
Main Results:
- Aged control rats showed increased accumulation of oxidative DNA damage products.
- Grape seed extract supplementation significantly inhibited the accumulation of 8-OHdG in aged rats.
- GSE treatment also reduced DNA-protein cross-links in the spinal cord and brain regions of aged rats.
Conclusions:
- Grape seed extract demonstrates a salubrious role in mitigating age-related oxidative DNA damage.
- GSE exhibits neuroprotective properties by reducing DNA damage in the aging central nervous system.
- These findings highlight GSE as a potential agent for combating age-associated neurodegenerative processes.