[Influence of B mating-type factor on recovery of nuclear types from dikaryons in Lentinula edodes]

This study determined a genetic basis for the phenomenon of significant asymmetric ratio of the two component nuclear types from dikaryons of Lentinula edodes via protoplast formation and regeneration by clarifying the influence of A or B mating-type factor on recovery of nuclear types from dikuaryons. The results revealed that B factor or some supposed genes linked to the B factor appeared to influence survival of dedikaryotized nuclei,whereas A factor had no apparent effect on this phenomenon. Specificity of B factor was shown to correlate with percentage survival of the component nuclear types recovered from both kinds of heterokaryons (A not equal to B not equal to and A = B not equal to). It can be put in an hierarchical order with respect to this function,the hierarchical order of these B factors was B1 > B3 > B4 > B2 between two tested strains.