Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Biological electron transfer: progress and future directions.

M A Cusanovich1, M S Caffrey

  • 1Department of Biochemistry, University of Arizona, Tucson.

Biochimica Et Biophysica Acta
|May 23, 1991
PubMed
Summary

Bacterial cytochromes are crucial for understanding biological electron transfer. New techniques like site-directed mutagenesis and NMR spectroscopy will soon clarify intramolecular and intracomplex electron transfer mechanisms.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Plasmon waveguide resonance spectroscopic evidence for differential binding of oxidized and reduced Rhodobacter capsulatus cytochrome c2 to the cytochrome bc1 complex mediated by the conformation of the Rieske iron-sulfur protein.

Biochemistry·2007
Same author

Binding of oxidized and reduced cytochrome c2 to photosynthetic reaction centers: plasmon-waveguide resonance spectroscopy.

Biochemistry·2004
Same author

Structure of cytochrome c' from Rhodobacter capsulatus strain St Louis: an unusual molecular association induced by bridging Zn ions.

Acta crystallographica. Section D, Biological crystallography·2004
Same author

Protein dynamics in the region of the sixth ligand methionine revealed by studies of imidazole binding to Rhodobacter capsulatus cytochrome c2 hinge mutants.

Biochemistry·2004
Same author

Rhodobacter capsulatus photoactive yellow protein: genetic context, spectral and kinetics characterization, and mutagenesis.

Biochemistry·2004
Same author

Amino acid sequences and distribution of high-potential iron-sulfur proteins that donate electrons to the photosynthetic reaction center in phototropic proteobacteria.

Journal of molecular evolution·2003

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biophysics

Background:

  • Bacterial cytochromes are vital for biological electron transfer.
  • Previous research elucidated factors in collision-dependent electron transfer (driving force, electrostatics, surface topology).
  • Limited knowledge exists regarding intramolecular and intracomplex electron transfer mechanisms.

Purpose of the Study:

  • To highlight the importance of bacterial cytochromes in biological electron transfer.
  • To identify key factors influencing intramolecular and intracomplex electron transfer.
  • To emphasize the need for further research into these processes.

Main Methods:

  • Review of existing literature on electron transfer kinetics.
  • Discussion of factors affecting intramolecular and intracomplex electron transfer.

Related Experiment Videos

  • Introduction of advanced techniques for mechanistic studies.

    • Main Results:

    • Established the significance of driving force, distance, orientation, media, and dynamics in electron transfer.
    • Identified a knowledge gap in understanding these factors for intramolecular and intracomplex reactions.
    • Highlighted the potential of new techniques to elucidate these mechanisms.

    Conclusions:

    • Bacterial cytochromes are essential models for studying electron transfer.
    • Further investigation is required to understand intramolecular and intracomplex electron transfer.
    • Site-directed mutagenesis and NMR spectroscopy are promising tools for future research.