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Real-time assay of immobilized tannase with a stopped-flow conductometric device
Fu-Shiang Chang1, Po-Chung Chen, Richie L C Chen
1Department of Bio-Industrial Mechatronics Engineering, College of Bioresources and Agriculture, National Taiwan University, Taipei, Taiwan.
Abstract:
A stopped-flow manifold was developed to assay and characterize immobilized tannase (EC 3.1.1.20). The immobilized enzyme reactor was inserted within the tube-type electrode pair (cell constant=103.2 cm(-1)) for a real-time conductometric measurement. Tris buffer (2 mM, pH=7.0) was used as the carrier for sensitivity improvement. The activities and kinetic parameters (Km values) for propyl gallate, methyl gallate and tannic acid were investigated.
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