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Updated: Aug 11, 2026
Detection of Bacteria Using Fluorogenic DNAzymes
Published on: May 28, 2012
Freezing induces biased results in the molecular detection of Flavobacterium columnare
Lotta-Riina Suomalainen1, Hilkka Reunanen, Ritva Ijäs
1Department of Biological and Environmental Science, University of Jyväskylä, P.O. Box 35, FIN-40014 University of Jyväskylä, Finland.
Abstract:
Specific PCR detection and electron microscopy of Flavobacterium columnare revealed the risk of false-negative results in molecular detection of this fish pathogen. Freezing and thawing destroyed the cells so that DNA was for the most part undetectable by PCR. The detection of bacteria was also weakened after prolonged enrichment cultivation of samples from infected fish.
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