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Expression of CD28 and CTLA4 on T cells in bone morrow of immune-mediated aplastic anemia mice
Zhenfang Liu1, Hanying Sun, Wenli Liu
1Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Abstract:
To investigate the expression and significance of CD28 and CTLA4 on T cells in bone marrow of aplastic anemia (AA) mice, in vitro bone marrow mononuclear cells (BMMNCs) were activated through being incubated with PHA (15 microg/mL). The expression of CD28 and CTLA4 on T cells incubated with or without PHA was detected by two-color flow cytometry. The expression of CD28 and CTLA4 was significantly increased after PHA stimulation. In the AA mice, the expression of CD28 with or without PHA stimulation was both higher than that in the normal mice (both P < 0.01), but the expression of CTLA4 with or without PHA stimulation showed no significant difference in comparison to that in the normal mice (both P > 0.05). In the AA mice, there were more activation and activated potential of T cells than the normal, and the abnormal expression of CD28 and CTLA4 may participate in immunological disorder mediated by T cells.
Insights
T cells in aplastic anemia (AA) mice show higher CD28 expression compared to normal mice. This altered expression of CD28 and CTLA4 on T cells may contribute to the immune dysregulation observed in AA.
Area of Science:
- Immunology
- Hematology
Background:
- Aplastic anemia (AA) is a rare bone marrow failure disorder.
- T cell-mediated immune responses are implicated in the pathogenesis of AA.
Purpose of the Study:
- To investigate the expression of CD28 and CTLA4 on T cells in the bone marrow of AA mice.
- To understand the role of these molecules in T cell activation and immune dysregulation in AA.
Main Methods:
- Bone marrow mononuclear cells (BMMNCs) from AA and normal mice were isolated.
- In vitro activation of BMMNCs using phytohemagglutinin (PHA).
- Two-color flow cytometry was used to detect CD28 and CTLA4 expression on T cells.
Main Results:
- PHA stimulation significantly increased CD28 and CTLA4 expression on T cells.
- AA mice exhibited significantly higher CD28 expression on T cells, with or without PHA stimulation, compared to normal mice.
- No significant difference in CTLA4 expression was observed between AA and normal mice, with or without PHA stimulation.
Conclusions:
- T cells in AA mice display enhanced activation and potential compared to normal mice.
- Abnormal CD28 expression on T cells in AA may play a role in the immunological disorder characteristic of the disease.
