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Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
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A reversible pH-driven DNA nanoswitch array.

Dongsheng Liu1, Andreas Bruckbauer, Chris Abell

  • 1Nanoscience Centre, University of Cambridge, 11 J J Thomson Avenue, Cambridge CB3 0FF, U.K. liuds@nanoctr.cn

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|February 9, 2006
PubMed
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Proton-fueled DNA nanomachines actuate using pH changes, altering their structure to move a fluorophore. This creates a nanoscale motion transducer, functioning as an optical "on-and-off" nanoswitch for potential biosensing applications.

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Area of Science:

  • Nanotechnology
  • Biochemistry
  • Surface Science

Background:

  • DNA nanomachines offer precise control at the nanoscale.
  • Proton (H+) concentration can be utilized for actuating molecular devices.

Purpose of the Study:

  • To develop a proton-fueled DNA nanomachine.
  • To create a reversible nanoscale actuator.
  • To transduce nanoscale motion into an optical signal.

Main Methods:

  • Surface immobilization of DNA nanomachines on a gold surface.
  • Reversible actuation via cycling solution pH between 4.5 and 9.
  • Labeling DNA with a fluorophore at the 3' end.
  • Monitoring fluorophore displacement using optical detection.

Main Results:

  • DNA nanomachines exhibited reversible conformational changes between four-stranded and double-stranded structures.
  • Nanoscale motion resulted in a displacement of at least 2.5 nm for the fluorophore.
  • The system functioned as an optical "on-and-off" nanoswitch.

Conclusions:

  • Proton-fueled DNA nanomachines can be reversibly actuated by pH cycling.
  • This system effectively transduces nanoscale mechanical work into an optical signal.
  • The developed nanoswitch demonstrates potential for biosensing and nanodevice applications.