Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene expression profiles in developing nephrons using Lim1 metanephric mesenchyme-specific conditional mutant mice.

You-Tzung Chen1, Akio Kobayashi, Kin Ming Kwan

  • 1Department of Molecular Genetics, University of Texas M. D. Anderson Cancer Center, Houston, Texas 77030, USA. ytchen@mdanderson.org

BMC Nephrology
|February 9, 2006
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author Correction: The transcriptional coactivator TAZ regulates reciprocal differentiation of T<sub>H</sub>17 cells and T<sub>reg</sub> cells.

Nature immunology·2026
Same author

Diagnostic Value of High-Resolution Ultrasound for Unilateral Digital Nerve Injuries in Small or Already Closed Wounds.

The journal of hand surgery Asian-Pacific volume·2026
Same author

Hippo Signaling Suppresses Cell Ploidy and Tumorigenesis through Skp2.

Cancer cell·2026
Same author

Combined anteversion in natural Asian hips is lower than conventional targets and is predominantly determined by femoral anteversion.

International orthopaedics·2026
Same author

Capsular reconstruction versus excision in anterolateral total hip arthroplasty: a prospective randomized controlled trial.

International orthopaedics·2026
Same author

Meet Our Editorial Board-Kin Ming Kwan, Chinese University of Hong Kong, Hong Kong, China.

Genesis (New York, N.Y. : 2000)·2026

Lim1 is crucial for kidney development. Studying Lim1 mutant kidneys identified 465 potential nephron-specific genes, aiding in understanding kidney formation and function.

Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • Lim1 is a homeobox gene vital for nephrogenesis, the process of kidney development.
  • Its expression is observed in key structures like the nephric duct and ureteric buds during kidney formation.
  • Loss of Lim1 function in the metanephric mesenchyme results in underdeveloped kidneys lacking nephrons.

Purpose of the Study:

  • To identify novel nephron-specific genes using Lim1 conditional mutant kidneys.
  • To leverage gene expression profiling to discover genes involved in kidney development.

Main Methods:

  • Utilized Affymetrix probe arrays to compare gene expression profiles of control and Lim1 conditional mutant kidneys.
  • Analyzed kidney tissues at two embryonic stages: E14.5 and E18.5.

Related Experiment Videos

  • Employed computational analysis to validate the enrichment of kidney-specific genes.

    • Main Results:

    • Identified 465 candidate nephron-specific genes with over 2-fold increased expression in control kidneys at E18.5.
    • Confirmed enrichment of kidney-specific genes through computational analysis.
    • Found 41 candidate genes at E14.5, including three linked to the Notch signaling pathway crucial for cell fate and patterning.

    Conclusions:

    • Lim1 conditional mutant kidneys represent a valuable resource for comprehensive gene expression studies.
    • This approach successfully identified novel nephron-specific genes, advancing the understanding of kidney development.