Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Transcription factors drive B cell development.

James Hagman1, Kara Lukin

  • 1Integrated Department of Immunology, National Jewish Medical and Research Center, 1400 Jackson Street, K516B, Denver, CO 80206, USA. hagmanj@njc.org

Current Opinion in Immunology
|February 9, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Leveraging Micro-Stories to Build Engagement, Inclusion, and Neural Networking in Immunology Education.

Frontiers in immunology·2019
Same author

Specific Recognition of Arginine Methylated Histone Tails by JMJD5 and JMJD7.

Scientific reports·2018
Same author

The transcription factors GATA2 and microphthalmia-associated transcription factor regulate Hdc gene expression in mast cells and are required for IgE/mast cell-mediated anaphylaxis.

The Journal of allergy and clinical immunology·2017
Same author

Critical Functions of IRF4 in B and T Lymphocytes.

Journal of immunology (Baltimore, Md. : 1950)·2017
Same author

Clipping of arginine-methylated histone tails by JMJD5 and JMJD7.

Proceedings of the National Academy of Sciences of the United States of America·2017
Same author

De Novo Mutations in EBF3 Cause a Neurodevelopmental Syndrome.

American journal of human genetics·2016

Key transcription factors PU.1, E2A, and early B cell factor (EBF) orchestrate B lymphocyte development. While PU.1 initiates B lymphopoiesis, EBF and Pax5 activate crucial B cell-specific genes and epigenetic modifications.

Area of Science:

  • Immunology
  • Molecular Biology
  • Developmental Biology

Background:

  • Transcription factors PU.1, E2A, and early B cell factor (EBF) are critical for early B lymphocyte development.
  • PU.1 initiates B lymphopoiesis but may not be essential at later stages.
  • E2A proteins are vital for B cell lineage determination, with E2A-deficient progenitors exhibiting pluripotency.

Purpose of the Study:

  • To elucidate the hierarchical roles of transcription factors in B cell development.
  • To understand the interplay between PU.1, E2A, EBF, and Pax5 in B lymphopoiesis.
  • To investigate the mechanisms by which EBF facilitates Pax5 function.

Main Methods:

  • Analysis of transcription factor roles in B lymphocyte development.
  • Investigating gene expression patterns during B cell differentiation.

Related Experiment Videos

  • Examining epigenetic modifications at target genes.

    • Main Results:

    • PU.1, E2A, and EBF are essential for the earliest stages of B cell development.
    • PU.1 and E2A are required for the expression of EBF.
    • EBF activates B cell-specific genes and facilitates Pax5 function through epigenetic changes.

    Conclusions:

    • A hierarchy of transcription factors, including PU.1, E2A, EBF, and Pax5, orchestrates B cell development.
    • EBF plays a crucial role in activating the B cell gene program and enabling Pax5-mediated epigenetic modifications.
    • These factors collectively ensure proper B lymphocyte lineage commitment and differentiation.