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Updated: Aug 11, 2026

A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro
Published on: May 17, 2017
[Genome-wide analysis for transcriptional regulation of chondrogenesis-related genes]
1Department of Regenarative Biology and Medicine, National Research Institute for Child Health and Development.
Abstract:
Transcriptional factor Sox9 plays a central role in development of chondrogenesis with stage specificity and in tissue specificity. Sox9 with a number of transcriptional cofactors is a critical regulator in genes such as COL2, chondorocyte specific gene. We report here our new large scale approaches : 1) Whole mount in situ hybridization (WISH), 2) High through-put human cDNA transfection assay. These genome-wide approaches have a possibility of discovery for new transcription factors in chondrogenesis.
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