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Alterations in novel candidate tumor suppressor genes, ING1 and ING2 in human lung cancer

Tetsuya Okano1, Akihiko Gemma, Yoko Hosoya

  • 1Fourth Department of Internal Medicine, Nippon Medical School, Tokyo, Japan.

Oncology Reports
|February 9, 2006
PubMed

Insights

Mutations in ING1 and ING2 genes were not found in lung cancer. However, ING1 expression increased with p53 mutations, while ING2 expression decreased, suggesting distinct roles in lung cancer progression.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • The ING1 gene regulates cell cycle, senescence, and apoptosis, acting as a tumor suppressor.
  • The ING2 gene, part of the ING family, also influences cellular processes.
  • Both ING1 and ING2 functions are linked to the activity of the p53 protein.

Purpose of the Study:

  • To investigate mutations in ING1 and ING2 genes in lung cancer.
  • To analyze the expression patterns of ING1 and ING2 in relation to p53 status in lung cancer.

Main Methods:

  • Screening for mutations using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and direct sequencing.
  • Quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) to assess gene expression levels.

Main Results:

  • No mutations were detected in the ING1 and ING2 genes in lung cancer cell lines or primary tumors.
  • ING1 expression was significantly upregulated in lung cancer cell lines with p53 mutations.
  • ING2 expression was downregulated in most lung cancer cell lines with p53 mutations.

Conclusions:

  • ING1 and ING2 genes do not appear to be mutated in lung cancer.
  • Altered expression of ING1 and ING2, particularly in relation to p53 status, suggests distinct roles in lung carcinogenesis.
  • ING2 may function as an independent tumor suppressor candidate in a p53-dependent manner.

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