Related Experiment Video
Updated: Aug 11, 2026

Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity
Published on: March 31, 2021
Continuous spin fractionation and characterization by size-exclusion chromatography for styrene-butadiene block
Xiaopeng Xiong1, John Eckelt, Bernhard A Wolf
1Department of Chemistry, Wuhan University, 430072 Wuhan, China.
Abstract:
Linear and star-shaped styrene-butadiene block copolymers synthesized by anionic polymerization of butadiene and styrene were fractionated by applying a newly developed large-scale fractionation technique, named continuous spin fractionation (CSF). Their molecular weight and polydispersity index (d=M(w)/M(n)) were measured with size-exclusion chromatography and static light scattering. For the linear triblock copolymer a fractionation via temperature variation turned out to be better suited than the usual isothermal procedure. The star-shaped polymer with the d value of 1.33 was fractionated in two CSF steps to get the targeted sample, which has a considerably more uniform structure and a narrower molecular weight distribution (d=1.11). The corresponding starting linear diblock copolymer was fractionated in one step reducing d from 1.68 to 1.17. With one set of simple laboratory equipment, 1kg polymer can be fractionated per day. Utilizing CSF, for the first time, we fractionated successfully the block copolymers.
More Related Videos
09:02Using Polystyrene-block-poly(acrylic acid)-coated Metal Nanoparticles as Monomers for Their Homo- and Co-polymerization
Published on: July 9, 2015
10:53Anionic Polymerization of an Amphiphilic Copolymer for Preparation of Block Copolymer Micelles Stabilized by π-π Stacking Interactions
Published on: October 10, 2016
Related Concept Videos
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...
Supercritical Fluid Chromatography
SFC utilizes a supercritical fluid mobile phase,...
Centrifugation
Optimizing Chromatographic Separations
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
Mass Spectrometry: Cycloalkene Fragmentation
Polymer Classification: Stereospecificity