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Updated: Jul 23, 2026

Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies
Published on: March 6, 2013
ThPTR2, a di/tri-peptide transporter gene from Trichoderma harzianum
J A Vizcaíno1, R E Cardoza, M Hauser
1Spanish-Portuguese Center of Agricultural Research (CIALE), Departamento de Microbiología y Genética, Universidad de Salamanca, Edificio Departamental lab 208, Plaza Doctores de la Reina s/n, 37007 Salamanca, Spain.
Researchers identified the ThPTR2 gene in Trichoderma harzianum, which codes for a peptide transporter. This gene is crucial for the fungus
Area of Science:
- Fungal genetics and molecular biology
- Biochemistry and transporter function
- Biocontrol and plant pathology
Background:
- Trichoderma species are vital biocontrol agents.
- Understanding their nutrient transport mechanisms is key to their efficacy.
- Peptide transporters play a role in fungal growth and interaction.
Purpose of the Study:
- To identify and characterize peptide transporter genes in Trichoderma harzianum.
- To investigate the role of the ThPTR2 gene in fungal physiology and mycoparasitism.
- To confirm ThPTR2 as a functional di/tri-peptide transporter.
Main Methods:
- Expressed Sequence Tag (EST) library generation and database analysis.
- Gene cloning, sequencing, and protein domain analysis.
- Gene expression analysis under various growth conditions (chitin, nitrogen sources, interaction with Botrytis cinerea).
- Functional analysis using gene overexpression in Trichoderma strains and dipeptide uptake assays.
Main Results:
- The gene ThPTR2, encoding a PTR family di/tri-peptide transporter with 12 transmembrane domains, was identified.
- ThPTR2 expression is highest on chitin, induced during mycoparasitism with Botrytis cinerea, and responsive to nitrogen availability.
- Overexpression of ThPTR2 significantly increased dipeptide (Leu-Leu) uptake, confirming its function.
- Homologous genes were found in other Trichoderma strains.
Conclusions:
- ThPTR2 is the first confirmed PTR family transporter gene in filamentous fungi.
- This transporter is involved in nutrient acquisition and the mycoparasitic interaction of Trichoderma.
- ThPTR2 represents a potential target for enhancing Trichoderma's biocontrol capabilities.
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