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Updated: Aug 11, 2026

Dissection and Culture of Mouse Embryonic Kidney
Published on: May 17, 2017
Expression analysis of the Epha1 receptor tyrosine kinase and its high-affinity ligands Efna1 and Efna3 during early
Shannon L Duffy1, Kirsten A Steiner, Patrick P L Tam
1Leukaemia Foundation Research Unit, Queensland Institute of Medical Research, Herston, Australia. Shannon.Duffy@qimr.edu.au
Abstract:
Interaction of Eph receptor tyrosine kinases with their membrane bound ephrin ligands initiates bidirectional signaling events that regulate cell migratory and adhesive behavior. Whole-mount in situ hybridization revealed overlapping expression of the Epha1 receptor and its high-affinity ligands ephrin A1 (Efna1) and ephrin A3 (Efna3) in the primitive streak and the posterior paraxial mesoderm during early mouse development. These results show complex and dynamic expression for all three genes with expression domains that are successively complementary, overlapping, and divergent.
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