Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ofloxacin, a bactericidal antibacterial.

J T Smith1

  • 1Department of Pharmaceutics, School of Pharmacy, University of London, UK.

Chemotherapy
|January 1, 1991
PubMed
Summary

Ofloxacin and other 4-quinolones uniquely target bacterial DNA synthesis, offering oral administration for severe infections. Resistance develops solely through chromosomal mutation, which may reduce bacterial pathogenicity and prevent transfer.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Estimation of <sup>90</sup>Sr and <sup>137</sup>Cs activity concentrations in Chornobyl wood: significance of factors and classical vs. machine learning methods.

Journal of environmental radioactivity·2025
Same author

A protocol for the radiological assessment for agricultural use of land in Ukraine abandoned after the Chornobyl accident.

Journal of environmental radioactivity·2025
Same author

The role of placental kisspeptin in trophoblast invasion and migration: an assessment in <i>Kiss1r</i> knockout mice, BeWo cell lines and human term placenta.

Reproduction, fertility, and development·2024
Same author

NIR Fluorescence lifetime macroscopic imaging with a time-gated SPAD camera.

Proceedings of SPIE--the International Society for Optical Engineering·2022
Same author

A novel therapy, using Ghrelin with pegylated G-CSF, inhibits brain hemorrhage from ionizing radiation or combined radiation injury.

Pharmacy & pharmacology international journal·2021
Same author

Thermoneutral conditions correct the obese phenotype in female, but not male, Kiss1r knockout mice.

Journal of thermal biology·2020

Area of Science:

  • Microbiology
  • Pharmacology
  • Genetics

Background:

  • Ofloxacin belongs to the 4-quinolone class of antibiotics.
  • Unlike many antibiotics, 4-quinolones target bacterial DNA synthesis.
  • They are effective for serious infections and can be administered orally.

Purpose of the Study:

  • To highlight the unique mechanisms of action and resistance of 4-quinolone antibacterials.
  • To emphasize the distinct properties of ofloxacin compared to other frontline antibiotics.

Main Methods:

  • The study focuses on the biochemical and genetic mechanisms of ofloxacin action.
  • It examines the pathways of bacterial resistance to 4-quinolones.

Main Results:

  • 4-quinolones inhibit bacterial DNA synthesis, distinct from cell wall or protein synthesis inhibition.
  • Resistance to 4-quinolones is primarily achieved through chromosomal mutation, not plasmid-mediated transfer.
  • Mutations conferring resistance may also decrease bacterial pathogenicity.
  • Resistance acquired through mutation is not transferable via conjugation.

Conclusions:

  • Ofloxacin and 4-quinolones possess unique antibacterial properties.
  • Their mechanism of action and resistance profile present a distinct advantage in combating bacterial infections.
  • The chromosomal basis of resistance limits horizontal gene transfer, potentially slowing the spread of resistance.

Related Experiment Videos