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Related Experiment Videos

Reverse gyrase in thermophilic eubacteria.

C Bouthier de la Tour1, C Portemer, R Huber

  • 1Laboratoire d'Enzymologie des Acides Nucléiques, URA 554 Centre National de la recherche Scientifique, Université Pierre et Marie Curie, Paris, France.

Journal of Bacteriology
|June 1, 1991
PubMed
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Reverse gyrase, an enzyme crucial for life at high temperatures, was found in Thermotogales eubacteria. This suggests its vital role in thermophilic organisms across different evolutionary kingdoms.

Area of Science:

  • Molecular Biology
  • Extremophile Research
  • Genomics

Background:

  • Thermophilic organisms thrive in extreme heat.
  • Reverse gyrase is an ATP-dependent type I topoisomerase.
  • This enzyme was previously found only in thermophilic archaebacteria.

Purpose of the Study:

  • To investigate the presence of reverse gyrase in Thermotogales.
  • To understand the distribution of reverse gyrase in high-temperature-living organisms.

Main Methods:

  • Isolation of DNA from Thermotogales strains.
  • Detection of reverse gyrase activity or gene presence.

Main Results:

  • Reverse gyrase was detected in four strains of Thermotogales.

Related Experiment Videos

  • Thermotogales are extremely thermophilic eubacteria.

    • Conclusions:

    • Reverse gyrase is present in both thermophilic archaebacteria and eubacteria.
    • This enzyme likely plays a fundamental role in enabling life at high temperatures, irrespective of evolutionary lineage.