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The priB gene encoding the primosomal replication n protein of Escherichia coli
1Department of Biochemistry, Stanford University School of Medicine, California 94305.
The Journal of Biological Chemistry
|July 5, 1991
Abstract:
The gene encoding protein n of the Escherichia coli primosome has been discovered in the rpsF-rpsR-rplI ribosomal protein operon and designated priB. The low copy number of PriB protein and the distinctive codon usage of its gene argue against its being a ribosomal protein. A strain which overproduces PriB was constructed and has been used to purify the protein to homogeneity. The overproduced protein behaves like that purified from wild-type cells.