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[Isolation and characterization of a DRE-binding transcription factor from Yinxin poplar (Populus alba x P. alba var.
Hong-Xia Qin1, Zhi-Ping Jia, Hai-Chao Zhang
1College of Life Science, Ningxia University, Yinchuan 750021, China.
Abstract:
Dehydration-Responsive Element Binding ( DREB) transcription factors, specifically binding with dehydration reponsive element (DRE), activate a variety of stress-responsive genes in plants under abiotic stresses (dehydration, high salt and low temperature). Using PCR and homologous EST search, we isolated a DREB-like gene from Yinxin poplar (Populus alba x P. alba var. pyramidalis) named PaDREB2. Yeast One-hybrid experiment demonstrated that PaDREB2 protein could function as a DREB transcription factor activating target gene expression by specifically binding to DRE cis-element. To study the expression pattern of PaDREB2, RT-PCR was carried out. And the results showed that PaDREB2 is induced by low temperature, drought and high salt.
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