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Measuring Lactase Enzymatic Activity in the Teaching Lab
Published on: August 6, 2018
[Relief effect of beta-galactosidase genetically engineered lactococcus lactis on the cell toxicity caused by
Xiang Liu1, Xiao-ying Lü, Qian Yu
1Department of Medical Technology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Objective:
To assess the relief effect of beta-galactosidase genetically engineered Lactococcus lactis on the cell toxicity caused by lactose in vitro.
Methods:
An in vitro toxic Caco-2 cell model caused by lactose was established to evaluate the relief effect of beta-galactosidase genetically engineered Lactococcus lactis. Cell morphological parameters and proliferation activity parameter were used.
Results:
The in vitro toxic Caco-2 cell model caused by lactose was successfully established; the genetically engineered Lactococcus lactis constructed in the authors' laboratory could enable the Caco-2 cell to have normal appearance with the presence of lactose and could improve the proliferation activity with the presence of high concentration of lactose (P < 0.01).
Conclusion:
The beta-galactosidase genetically engineered Lactococcus lactis has significant relief effect on the cell toxicity caused by lactose in vitro, which lays a foundation for food-grade alternation of this bacterium.
