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Related Experiment Video

Updated: Jul 23, 2026

Human Pancreatic Islet Isolation: Part II: Purification and Culture of Human Islets
08:35

Human Pancreatic Islet Isolation: Part II: Purification and Culture of Human Islets

Published on: May 26, 2009

Modified two-layer preservation method (M-Kyoto/PFC) improves islet yields in islet isolation.

H Noguchi1, M Ueda, Y Nakai

  • 1Diabetes Research Institute Kyoto, Kyoto University Graduate School of Medicine, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan. noguchih@kuhp.kyoto-u.ac.jp

American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons
|February 14, 2006
PubMed
Summary

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A new M-Kyoto solution enhances islet yield in transplantation by inhibiting trypsin and minimally affecting collagenase, outperforming UW solution in pancreas preservation for type I diabetes treatment.

Area of Science:

  • Transplantation immunology
  • Organ preservation
  • Diabetes research

Background:

  • Islet allotransplantation is a key treatment for type I diabetes, aiming for insulin independence.
  • The two-layer method (TLM) using oxygenated perfluorochemical (PFC) and UW solution improves pancreas preservation compared to simple cold storage.
  • UW solution, while effective, inhibits crucial enzymes like Liberase, potentially impacting islet isolation efficiency.

Purpose of the Study:

  • To evaluate a novel preservation solution, M-Kyoto solution, for pancreas preservation in islet transplantation.
  • To compare the efficacy of M-Kyoto solution against UW solution, particularly concerning enzyme activity and islet yield.
  • To assess the components of M-Kyoto solution, trehalose and ulinastatin, for their cytoprotective and enzyme-inhibiting properties.

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A Method for Murine Islet Isolation and Subcapsular Kidney Transplantation
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Effective Isolation of Functional Islets from Neonatal Mouse Pancreas
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Effective Isolation of Functional Islets from Neonatal Mouse Pancreas

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Related Experiment Videos

Last Updated: Jul 23, 2026

Human Pancreatic Islet Isolation: Part II: Purification and Culture of Human Islets
08:35

Human Pancreatic Islet Isolation: Part II: Purification and Culture of Human Islets

Published on: May 26, 2009

A Method for Murine Islet Isolation and Subcapsular Kidney Transplantation
17:42

A Method for Murine Islet Isolation and Subcapsular Kidney Transplantation

Published on: April 13, 2011

Effective Isolation of Functional Islets from Neonatal Mouse Pancreas
07:04

Effective Isolation of Functional Islets from Neonatal Mouse Pancreas

Published on: January 6, 2017

Main Methods:

  • Porcine islet isolation was performed using M-Kyoto solution/PFC and UW solution/PFC.
  • Pancreas ATP content was measured to assess energy source differences.
  • Trypsin and collagenase activity were measured in both M-Kyoto and UW solutions during the digestion step.

Main Results:

  • The M-Kyoto/PFC group showed a significantly higher islet yield compared to the UW/PFC group.
  • No significant difference in pancreas ATP content was observed between the groups.
  • M-Kyoto solution demonstrated significant trypsin inhibition and less inhibition of collagenase digestion compared to UW solution.

Conclusions:

  • M-Kyoto solution offers advantages in islet transplantation due to its trypsin inhibition and minimal collagenase inhibition.
  • The enhanced islet yield observed with M-Kyoto solution is not attributable to differences in energy sources.
  • M-Kyoto solution is now utilized for clinical islet transplantation from non-heart-beating donors, improving outcomes for type I diabetes patients.