Effect of naltrexone on stress-induced bioactive prolactin release in aging male rats

Effects of the opiate receptor antagonist naltrexone (NALT, 10 mg/kg) on basal and novel environment stress-induced prolactin (PRL) release were examined in young (3-5 month) and old (22-24 month) male Copenhagen-Fischer 344 rats. Radioimmunoassay and Nb2 lymphoma bioassay were used to determine plasma immunoreactive (ir-) and bioactive (bio-) PRL levels, respectively. Although basal plasma irPRL levels were greater, bioPRL levels were significantly lower in old as compared to young rats. NALT induced significant decreases in basal plasma ir- and bioPRL concentrations in young rats, but had little or no effect on irPRL or bioPRL levels in old rats. Stress-induced elevations in irPRL levels were similar, but increases in bioPRL levels were attenuated in vehicle-treated old as compared to young rats. NALT pretreatment blocked stress-induced ir- and bioPRL release in both age groups. These results indicate that the role of endogenous opioid peptides (EOP) in regulating basal PRL secretion appears to be diminished, whereas EOP continue to be involved in stress-induced PRL release in the aging animal.