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Related Experiment Videos

Laser-induced bubbles in living cells.

Dmitri O Lapotko1

  • 1Laser Cytotechnology Laboratory, Luikov Heat and Mass Transfer Institute, Minsk 220072, Belarus. ld@hmti.ac.by

Lasers in Surgery and Medicine
|February 14, 2006
PubMed
Summary

Laser-activated micro- and nano-bubbles (LAB) offer a sensitive, non-toxic method for analyzing individual cell functions. This universal approach can monitor cell health, whether intact or nanoparticle-treated.

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Area of Science:

  • Cellular biology
  • Biophysics
  • Optical methods

Background:

  • Laser-activated micro- and nano-bubbles (LAB) show potential as cellular probes.
  • LABs are non-toxic and can measure functional cell properties.

Purpose of the Study:

  • To investigate LABs as universal, sensitive, and non-toxic probes for individual cell analysis.
  • To assess the feasibility of using LABs for monitoring cell physiological states.

Main Methods:

  • Induction of LABs in living blood and tumor cells using pulsed laser radiation (532 nm, 10 ns).
  • Detection and imaging of LABs via microscopy and flow cytometry.
  • Utilizing the thermal lens optical method for LAB detection.
  • Employing light-absorbing nanoparticles conjugated to monoclonal antibodies to enhance specificity and sensitivity.

Main Results:

  • LABs exhibited lifetimes of 0.02-10 microseconds and maximal diameters of 0.44-100 micrometers.
  • LAB parameters (thresholds, probabilities) correlated with the physiological state of cells.
  • LAB cytometry demonstrated increased specificity and sensitivity with nanoparticle conjugation.

Conclusions:

  • LABs represent a universal phenomenon applicable to monitoring individual cells.
  • This technique is suitable for both intact and nanoparticle-treated cells, offering sensitive and non-invasive analysis.

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