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[Relation between mutant p53 and multidrug resistance in gastric cancer]

Xin-you Xie1, Ya-jun Tan, Yong-liang Zhu

  • 1Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou 310016, China.

Zhejiang Da Xue Xue Bao. Yi Xue Ban = Journal of Zhejiang University. Medical Sciences
|February 14, 2006
PubMed
Abstract

Insights

Mutant p53 genes are linked to multidrug resistance in gastric cancer. This study found mutant p53 increases resistance to certain chemotherapy drugs, impacting treatment effectiveness.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Gastric cancer is a significant global health concern.
  • Multidrug resistance (MDR) is a major challenge in gastric cancer treatment.
  • The role of mutant p53 in gastric cancer MDR requires further elucidation.

Purpose of the Study:

  • To investigate the association between mutant p53 expression and multidrug resistance in gastric cancer cells.
  • To compare the chemotherapeutic sensitivity of gastric cancer cells with and without mutant p53.

Main Methods:

  • Gastric cancer cell line SGC-7901 was modified to express mutant p53 (mp53) and mp53+sv40Tag.
  • Messenger RNA (mRNA) levels of MDR-1 were analyzed using RT-PCR.
  • Cellular sensitivity to chemotherapeutic agents (5-Fu, ADM, CDDP) was assessed using the MTT assay.

Main Results:

  • Gastric cancer cells expressing mutant p53 exhibited elevated MDR-1 mRNA levels compared to controls.
  • Cells with mutant p53 showed increased sensitivity to 5-Fluorouracil (5-Fu) and Doxorubicin (ADM) but not Cisplatin (CDDP).
  • The co-expression of mp53 and SV40 Tag did not significantly alter sensitivity to ADM compared to mp53 alone.

Conclusions:

  • Mutant p53 gene expression is associated with multidrug resistance in gastric cancer.
  • The findings suggest a complex interplay between mutant p53 and response to specific chemotherapeutic agents in gastric cancer.
  • Targeting mutant p53 pathways may offer novel therapeutic strategies for overcoming drug resistance in gastric cancer.

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